samespots v4 5  (TotalLab Ltd)

Journal: International Journal of Molecular Sciences

Article Title: Changes in the Proteome of Medicago sativa Leaves in Response to Long-Term Cadmium Exposure Using a Cell-Wall Targeted Approach

doi: 10.3390/ijms19092498

Figure Lengend Snippet: PCA analysis of the gel-based spot intensity data from the three cell wall protein-enriched fractions. ( A ) CaCl 2 ; ( B ) EGTA; ( C ) LiCl. Statistical analysis was done with SameSpots software (TotalLab). Blue dots represent the four biological replicates of the control. Pink dots represent the four biological replicates of Cd-exposed samples. Grey and red numbers correspond to spot numbers considered for the statistical analysis.

Article Snippet: SameSpots software v4.5 (TotalLab, Newcastle upon Tyne, UK) was used for the relative quantitative image analysis.

Techniques: Software

Journal: International Journal of Molecular Sciences

Article Title: Changes in the Proteome of Medicago sativa Leaves in Response to Long-Term Cadmium Exposure Using a Cell-Wall Targeted Approach

doi: 10.3390/ijms19092498

Figure Lengend Snippet: Illustration of 2D DIGE from the cell wall protein-enriched CaCl 2 fraction from leaves of M. sativa . Extractions were performed in four replicates. Proteins were pre-labelled with different CyDye to enable relative quantitative protein analysis. Labelled samples were loaded onto Immobiline TM DryStrip 3–10 NL, 24 cm (GE Healthcare) followed by migration on HPE TM Large Gel NF-12.5% (Serva Electrophoresis GmbH). Indicated spots were selected for picking based on statistical parameters calculated by the SameSpots software (TotalLab). Images of 2D DIGE from EGTA and LiCl fractions are provided in . MW = molecular weight.

Article Snippet: SameSpots software v4.5 (TotalLab, Newcastle upon Tyne, UK) was used for the relative quantitative image analysis.

Techniques: Migration, Electrophoresis, Software, Molecular Weight